Foundation for Advanced Education in the Sciences

Workshop on Hydrodynamic and Thermodynamic Analysis of Macromolecules with SEDFIT and SEDPHAT

This workshop will review strategies for hydrodynamic and thermodynamic analysis of sedimentation equilibrium and sedimentation velocity analytical ultracentrifugation (AUC), dynamic light scattering (DLS), and isothermal titration calorimetry (ITC) for the characterization of biological macromolecules in solution.

The workshop will start on Monday morning and end on Friday at noon. On the first day, we will provide an introduction into the experimental aspects of AUC, DLS and ITC. This part will take place in the laboratory. We will review the basic operation of the instruments, choice of buffers, and detection systems, and experimental design dependent on the system under study. For AUC, assembling the AUC cells and operating the XLA/I will be discussed in detail. The following days, will cover many advanced topics of theory, data analysis and experimental setup. These lectures are accompanied by practical computer exercises with the software.

The workshop is aimed at researchers with at least limited experience in AUC, DLS and/or ITC. An integral part of the workshop is the presentation by the participants of their systems of interest and informal discussion of data.

REGISTER NOW